Leave a margin of at least 38 mm 1. Leave a margin of at least 25 mm 1 inch from the top, bottom and right edges.
Great topic — surprised to see no comments so far. Below are my comments, point by point. In fact, I consider it misleading at best in vivo, the differentiation of MSCs is NOT their major mechanism of action and inaccurate at worst many studies have provided evidence that MSCs, from a variety of tissue sources, are in fact pluripotent — yes, I know this is a contentious topic.
I agree, but I like the previous points, this deals mostly with semantics and does not necessarily reflect the clinical utility of the cells, as such, I would not consider this point a priority. For me, the topic of fresh vs. While I think this is a topic that needs further study, there are a couple of reasons why I think we could be chasing our tails.
First, many CD markers are inducible, as such using them to define a cell type will only be useful under specific conditions.
Second, we do not know the function of many of these markers. I wholeheartedly agree with the points made and would go a step further. Stem cells from any given tissue represent a heterogenous population.
I realize this will add significant complexity studying populations of cells rather than individual cell lines but I believe the clinical payoff s will be worth the effort. Bottom line, organizations like the ISCT should be the vanguard of this technology, but resources should be prioritized to clinically-relevant topics rather than semantics.
I agree that further discussion is needed. Consensus would be nice, but realistically, forget about it and just be as clear and consistent as you can be. Thank you of leading us into the conversation with your comments.
Regarding your points 1 and 2 nomenclature. I agree that naive MSCs should be on the list, since these would be the parent cells derived from the donor. I think a problem here is that we are working with cultured MSCs, which are a contrivance or an artefact produced by in vitro manipulation and expansion.
We can define naive MSCs, but does that category have value since it is a theoretical construct that evaporates as we try to capture it or might be changed by our attempts to examine it?
You got a couple of issues here.
You got a mixed population of multipotent, unipotent, null potent cells, aka MSCs in culture exposed to differentiation factors for weeks followed by non-quantitative readouts.
Problems with interpreting the data: In vitro assays could be run on replicate clonal populations,tightened up and made quantitative. We only defer to in vitro assays when in vivo assays fail us.
For human pluripotent cells we can only perform teratoma assays which is the best data, but is a pale comparison to the mouse. For expanded MSCs, Caplan has his in vitro osteogenic differentiation assay. True, it tests only osteogenesis, but it can be quantitative.
Again the problem of purified input populations exists. From my desire to move from preclinical into clinical trials, I applied to PACT and had a clinical translation advisory team assigned to evaluate my MSC data package.
Of course, the rationale they gave me for using banked cells was pretty good, and they are PACT: Based upon this input, I suggest that cryopreserved MSCs be considered as additional category. I agree that at CD and CD should be considered to be added to the list.
Its pretty clear that different culture conditions, isolation methods and donors produce different populations of cultured MSCs.
It is no great leap to assume that MSCs are affected by tissue environments, cell culture substrates and the oxygen tension. MSCs are different from different age donors, too.The Difference Between A Dissertation and Thesis.
Many Universities and accredited educational institutions clarify the difference between a dissertation and thesis paper in this way, a dissertation is written as an application to for acceptance into a faculty in their field of study, while a thesis is a report on a particular area of study.
Logistic Regression in Dissertation & Thesis Research What are the odds that a year-old, single woman who wears glasses and favors the color gray is a librarian? 0 Responses to “Should You Write a Master's Thesis?” Brent Wittmeier October 15, I’m one of those Regent students who successfully completed a thesis as part of my “vocational discernment,” as they tend to call things there.
The main difference between a thesis and a dissertation is when they are completed. The thesis is a project that marks the end of a master’s program, while the dissertation occurs during doctoral study.
Format for a quantitative research proposal. I teach quantitative research proposal writing to public health students. By using lectures, a case study and discussion of presentations of the students own research proposals, I try to familiarize them with the contents of a research proposal .
If you’re contemplating graduate school, you may have heard that a comprehensive paper is required to graduate, and you likely wonder what exactly is the difference between a thesis and a dissertation.